interferon β trif antibody  (Novus Biologicals)

Journal: Frontiers in Pharmacology

Article Title: Naoxintong capsule decreases circulating exosomes of miR-382-5p to protect LPS-induced vascular endothelial cell injury by targeting STC1 in vitro

doi: 10.3389/fphar.2026.1655883

Figure Lengend Snippet: RNA-seq of HMEC-1. (A) Volcano plots of DEGs (n = 5). (B) KEGG enrichment bubble plots of DEGs (n = 5). (C) DEGs related to vascular endothelial injury. (D) The relative mRNA expression levels of vascular endothelial injury-related genes verified by RT-qPCR (n = 3). (E) The relative mRNA expression levels of TLR4, TRIF and p65 were detected by RT-qPCR (n = 3). (F) The expression protein levels of TLR4, TRIF and p-p65/p65 were detected by Western blot (n = 3). Values are expressed as mean ± SD. * p < 0.05, ** p < 0.01. The screening criteria for DEGs were defined as FC ≥ 2 or ≤0.5, FDR < 0.05 and p -value <0.05.

Article Snippet: After transfer, the membranes were blocked with sealing liquid and incubated for 2 h. Following blocking, membranes were incubated with primary antibodies against β-actin (dilution 1:1,000, ab8227, Abcam), ALIX (dilution 1:1,000, ab275377, Abcam), TSG101 (dilution 1:1,000, ab125011, Abcam), CD9 (dilution 1:1,000, ab236630, Abcam), Bax (dilution 1:1,000, ab32503, Abcam), Bcl-2 (dilution 1:1,000, ab32124, Abcam), Cleaved caspase 3 (dilution 1:500, ab32042, Abcam), TLR4 (dilution 1:1,000, TA382568, OriGene), TRIF (dilution 1:1,000, TA382523, OriGene), NF-κB p65 (dilution 1:1,000, TA385159, OriGene), Phospho-NF-κB p-p65 (dilution 1:1,000, TA380848, OriGene), STC1 (dilution 1:5000, ab229477, Abcam) at 4 °C overnight.

Techniques: RNA Sequencing, Expressing, Quantitative RT-PCR, Western Blot

Journal: Frontiers in Pharmacology

Article Title: Naoxintong capsule decreases circulating exosomes of miR-382-5p to protect LPS-induced vascular endothelial cell injury by targeting STC1 in vitro

doi: 10.3389/fphar.2026.1655883

Figure Lengend Snippet: Schematic diagram illustrated that Naoxintong Capsule protected vascular endothelial cell from injury and dysfunction by downregulating miR-382-5p in circulating exosomes, potentially via targeting STC1 and inhibiting the TLR4/TRIF/NF-κB pathway.

Article Snippet: After transfer, the membranes were blocked with sealing liquid and incubated for 2 h. Following blocking, membranes were incubated with primary antibodies against β-actin (dilution 1:1,000, ab8227, Abcam), ALIX (dilution 1:1,000, ab275377, Abcam), TSG101 (dilution 1:1,000, ab125011, Abcam), CD9 (dilution 1:1,000, ab236630, Abcam), Bax (dilution 1:1,000, ab32503, Abcam), Bcl-2 (dilution 1:1,000, ab32124, Abcam), Cleaved caspase 3 (dilution 1:500, ab32042, Abcam), TLR4 (dilution 1:1,000, TA382568, OriGene), TRIF (dilution 1:1,000, TA382523, OriGene), NF-κB p65 (dilution 1:1,000, TA385159, OriGene), Phospho-NF-κB p-p65 (dilution 1:1,000, TA380848, OriGene), STC1 (dilution 1:5000, ab229477, Abcam) at 4 °C overnight.

Techniques:

Journal: Medical Oncology (Northwood, London, England)

Article Title: Genistein enhances TLR3-mediated apoptosis and immune signaling in breast cancer cells

doi: 10.1007/s12032-025-02856-5

Figure Lengend Snippet: IF detection of TLR-3, TRIF, IRF3, and p-NF-kB in breast cancer cells treated with Poly I:C, GEN or GEN + Poly I:C (a) Representative images of MCF-7 and (b) MDA-MB-231 cells under each indicated treatment. TLR-3, TRIF, IRF3, and p-NF-kB are stained in green, while nuclei are counterstained with DAPI (blue). Scale bars are shown in each panel

Article Snippet: To determine the levels of TLR3 (sc-32232, Santa Cruz), TRIF (sc-514384, Santa Cruz), IRF3 (sc-33641, Santa Cruz), p-NF-kB (3033, Cell signaling), and AP1 (MA5-15,172; Thermo Scientific) proteins in MCF-7 and MDA-MB-231 cells with the combination of GEN, Poly I:C, and GEN + Poly I:C, 1 × 10 5 cells were seeded into T75 flasks.

Techniques: Staining

Journal: Nutrients

Article Title: Effects of Soy Protein on Liver and Adipose Tissue Inflammation and Gut Microbiota in Mice Fed with Ketogenic Diets

doi: 10.3390/nu17152428

Figure Lengend Snippet: Hepatic ( A ) TLR4, ( B ) MyD88, ( C ) TRIF; and WAT ( D ), TLR4 ( E ), MyD88 ( F ) TRIF relative protein expression in mice. Values represent means ± SEM and n = 6–9. One-way ANOVA followed by Tukey’s multiple range test analysis, with lower-case letters denoting significantly different data groups in each panel. TLR4, toll-like receptor 4; MyD88, myeloid differentiation primary response 88; TRIF, TIR-domain-containing adapter-inducing interferon-β; C, control diet; KD, ketogenic diet; KS, ketogenic + soy protein diet.

Article Snippet: The membranes were than washed with phosphate-buffered saline/Tween-20 and incubated with an anti-toll-like receptor 4 (TLR4) antibody (monoclonal antibody to TLR4, IMG-5031A, Novus Biologicals, Centennial, CO, USA), anti-myeloid differentiation primary response 88 (MyD88) antibody (MyD88, D80F5 rabbit mAb, Cell Signaling Technology, Danvers, MA, USA), anti-TIR-domain-containing adapter-inducing interferon-β (TRIF) antibody (TRIF/TICAMI antibody, N8120-13810, Novus Biologicals, Centennial, CO, USA), anti-NLR family pyrin domain containing 3 (NLRP3) antibody (NLRP3 antibody [NBP2-1244], Novus Biologicals, US), anti-caspase-1 (p20) antibody (caspase-1 [p20] antibody, AG-20B-0042-C100, AdipoGen, San Diego, USA), FGF21 antibody (FGF21 antibody [EPR8314(2)], Abcam, Cambridge, MA, USA) and secondary antibodies (horser adish peroxidase [HRP] donkey anti-rabbit immunoglobulin G [IgG] antibody 406401; HRP goat anti-mouse IgG antibody 405306, Biolegend, San Diego, CA, USA).

Techniques: Expressing, Control